Journal: RSC Advances

Article Title: Replicating the post-chemotherapy tumor microenvironment via biomimetic scaffolds to regulate MSC differentiation

doi: 10.1039/d6ra01031h

Figure Lengend Snippet: Biological characterizations of DOX-MSNCaP@PLGA-collagen and PLGA-collagen constructs. The accumulate release of DOX from DOX-MSNCaP@PLGA-collagen in PBS buffer with different pH (A). Cytotoxic assessment of MG63 cells after being cultured in scaffolds via live and dead staining. Cells were seed in the meshes and incubated in DMEM at pH 7.4 or pH 6.5 (B). Fluorescence microscopic observation on the intracellular distribution of DOX in MG63 cells after incubation with DOX-MSNCaP@PLGA-collagen scaffolds for 12 hours. Cell nucleus was represented blue fluorescence; DOX was represented red fluorescence; actin cytoskeleton was represented green fluorescence (C). DNA laddering analysis on the severity of DNA fragmentation in MG63 cells upon treatment of DOX-MSNCaP@PLGA-collagen for 12 hours (I: PLGA-collagen at pH 7.4, II: DOX-MSNCaP@PLGA-collagen at pH 7.4, III: DOX-MSNCaP@PLGA-collagen at pH 6.5) (D).

Article Snippet: Different types of cells were utilized in this work, including human bone marrow-derived mesenchymal stem cells (MSCs, passage 4) obtained from LONZA (MD, USA), and the human osteosarcoma cell line (MG63) purchased from Procell Life Science & Technology Co., Ltd (China; Catalog No. CL-0157).

Techniques: Construct, Cell Culture, Staining, Incubation, Fluorescence, DNA Laddering

Journal: RSC Advances

Article Title: Replicating the post-chemotherapy tumor microenvironment via biomimetic scaffolds to regulate MSC differentiation

doi: 10.1039/d6ra01031h

Figure Lengend Snippet: BrdU staining of MG63 upon treatment of DOX-MSNCaP@PLGA-collagen at pH 7.4 or pH 6.5. Scale bar: 50 µm.

Article Snippet: Different types of cells were utilized in this work, including human bone marrow-derived mesenchymal stem cells (MSCs, passage 4) obtained from LONZA (MD, USA), and the human osteosarcoma cell line (MG63) purchased from Procell Life Science & Technology Co., Ltd (China; Catalog No. CL-0157).

Techniques: BrdU Staining

Journal: RSC Advances

Article Title: The lysine degradation pathway analyzed with 1 H-NMR-targeted metabolomics of MG63 cells on poly( l -lactide)-based scaffolds

doi: 10.1039/d5ra05954b

Figure Lengend Snippet: Representative 1 H NMR spectra of MG63 cell metabolites after being cultured on different types of PLLA. The cells were seeded on PLLA, PLLA-Cel, PLLA-Col, and PLLA-Cel-Col for 7 days. Peak assignments: 1 – lactic acid, 2 – 3-hydroxy- l -proline, 3 – glucosylgalactosylhydroxylysine, 4 – lysine, 5 – glucosylproline, 6 – pyruvic acid, 7 – succinic acid, 8 – α-ketoglutaric acid, 9 – proline, 10 – 5-hydroxylysine, 11 – oxaloacetic acid, 12 – acetyl-CoA, 13 – FADH 2 , 14 – 2-phosphoglyceric acid, 15 – cAMP, 16 – 3-phosphoglyceric acid, 17 – phosphoenolpyruvic acid, 18 – NADH. Unassigned peaks represent unknown compounds not in the HMDB database, residual solvent signals, or overlapping signals. The water signal region (4.6–5.0 ppm) was excluded from analysis to minimize interference.

Article Snippet: The MG63 osteosarcoma cell line (ATCC CRL1427, USA) was cultured in a sterile NuncTM EasyFlaskTM 75 (Thermo Fisher Scientific, China) using DMEM (Gibco®, USA) enriched with 10% fetal bovine serum (FBS), 2% HEPES buffer, 1% MEM non-essential amino acids (NEAAs), and 1% PenStrep (Gibco®, USA).

Techniques: Cell Culture, Solvent

Journal: RSC Advances

Article Title: The lysine degradation pathway analyzed with 1 H-NMR-targeted metabolomics of MG63 cells on poly( l -lactide)-based scaffolds

doi: 10.1039/d5ra05954b

Figure Lengend Snippet: Multivariate analyses of metabolomic profiles of MG63 cells cultured on PLLA and its composites, including PLLA-Cel, PLLA-Col, and PLLA-Cel-Col, for 7 days. Each data point represents a sample, with colors indicating the scaffold type: (A) principal component analysis (PCA) scores plot and (B) partial least-squares discriminant analysis (PLS-DA) scores plot.

Article Snippet: The MG63 osteosarcoma cell line (ATCC CRL1427, USA) was cultured in a sterile NuncTM EasyFlaskTM 75 (Thermo Fisher Scientific, China) using DMEM (Gibco®, USA) enriched with 10% fetal bovine serum (FBS), 2% HEPES buffer, 1% MEM non-essential amino acids (NEAAs), and 1% PenStrep (Gibco®, USA).

Techniques: Cell Culture

Journal: RSC Advances

Article Title: The lysine degradation pathway analyzed with 1 H-NMR-targeted metabolomics of MG63 cells on poly( l -lactide)-based scaffolds

doi: 10.1039/d5ra05954b

Figure Lengend Snippet: Metabolic pathway enrichment analysis of MG63 cells cultured on PLLA, PLLA-Cel, PLLA-Col, and PLLA-Cel-Col for 7 days.

Article Snippet: The MG63 osteosarcoma cell line (ATCC CRL1427, USA) was cultured in a sterile NuncTM EasyFlaskTM 75 (Thermo Fisher Scientific, China) using DMEM (Gibco®, USA) enriched with 10% fetal bovine serum (FBS), 2% HEPES buffer, 1% MEM non-essential amino acids (NEAAs), and 1% PenStrep (Gibco®, USA).

Techniques: Cell Culture

Journal: RSC Advances

Article Title: The lysine degradation pathway analyzed with 1 H-NMR-targeted metabolomics of MG63 cells on poly( l -lactide)-based scaffolds

doi: 10.1039/d5ra05954b

Figure Lengend Snippet: Effect of l -lysine concentration on total protein content in MG63 osteoblast-like cells.

Article Snippet: The MG63 osteosarcoma cell line (ATCC CRL1427, USA) was cultured in a sterile NuncTM EasyFlaskTM 75 (Thermo Fisher Scientific, China) using DMEM (Gibco®, USA) enriched with 10% fetal bovine serum (FBS), 2% HEPES buffer, 1% MEM non-essential amino acids (NEAAs), and 1% PenStrep (Gibco®, USA).

Techniques: Concentration Assay

Journal: RSC Advances

Article Title: The lysine degradation pathway analyzed with 1 H-NMR-targeted metabolomics of MG63 cells on poly( l -lactide)-based scaffolds

doi: 10.1039/d5ra05954b

Figure Lengend Snippet: Selected metabolites in the lysine degradation pathway. (A) The initial steps of the lysine degradation pathway and (B) the metabolite concentrations from MG63 cells cultured on PLLA, PLLA-Cel, PLLA-Col, and PLLA-Cel-Col for 7 days. The bar graph illustrating the mean ± SD ( n = 3) of each metabolite concentration (μM) is derived from 1 H NMR metabolomic data. The paired bars indicate a comparison of means; (★) and (★★), and (★★★) indicate significant differences when p < 0.05, 0.01, and 0.001, respectively.

Article Snippet: The MG63 osteosarcoma cell line (ATCC CRL1427, USA) was cultured in a sterile NuncTM EasyFlaskTM 75 (Thermo Fisher Scientific, China) using DMEM (Gibco®, USA) enriched with 10% fetal bovine serum (FBS), 2% HEPES buffer, 1% MEM non-essential amino acids (NEAAs), and 1% PenStrep (Gibco®, USA).

Techniques: Cell Culture, Concentration Assay, Derivative Assay, Comparison